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anti phospho slp76  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti phospho slp76
    Anti Phospho Slp76, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+slp76/pmc12629815-383-6-31
    Average 86 stars, based on 1 article reviews
    anti phospho slp76 - by Bioz Stars, 2026-09
    86/100 stars

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    other:

    Article Title: TULA-2 Deficiency Enhances Platelet Functional Responses to CLEC-2 Agonists
    Article Snippet: Anti-pSyk Y352, anti-pSyk Y525/Y526, anti-pPLCγ2 Y759, anti-pPLCγ2 Y1217, and anti-SLP76 were all purchased from Cell Signaling Technology (Beverly, MA).


    Article Title: Antibody-gamma/delta T cell receptors targeting GPC2 regress neuroblastoma with low antigen density.
    Article Snippet: The primary antibodies included anti-Phospho-PLCγ1 (#2821S), anti-Phospho-SLP76 (#14745S), anti-Phospho-LAT (#3584S), anti-Phospho-ZAP70 (#2701S), anti-Phospho-mTOR (#5536), anti-GAPDH (#2118S), anti-Histone H4 (#2592S), anti-PLCγ1 (#2822S), anti-SLP76 (#4958S), anti-ZAP70 (#2705S), e4 Cell Reports Medicine 6, 102378, October 21, 2025 Article ll OPEN ACCESS anti-mTOR (#2983), and anti-LAT (#45533S) from Cell Signaling Technology; and anti-NFATc1 (#649602) and anti-NFκB p65 (#653002) from Biolegend.

    Article Title: Antibody-gamma/delta T cell receptors targeting GPC2 regress neuroblastoma with low antigen density
    Article Snippet: The primary antibodies included anti-Phospho-PLCγ1 (#2821S), anti-Phospho-SLP76 (#14745S), anti-Phospho-LAT (#3584S), anti-Phospho-ZAP70 (#2701S), anti-Phospho-mTOR (#5536), anti-GAPDH (#2118S), anti-Histone H4 (#2592S), anti-PLCγ1 (#2822S), anti-SLP76 (#4958S), anti-ZAP70 (#2705S), anti-mTOR (#2983), and anti-LAT (#45533S) from Cell Signaling Technology; and anti-NFATc1 (#649602) and anti-NFκB p65 (#653002) from Biolegend.

    Incubation:

    Article Title: T-cell expression of Bruton’s tyrosine kinase promotes autoreactive T-cell activation and exacerbates aplastic anemia
    Article Snippet: .. The blots were incubated with anti-phospho-Btk (Tyr223) (5082; CST), anti-Btk (3532; 8547; CST), anti-Btk (sc28387; Santa Cruz), anti-PLCγ1 Y183 (14008; CST), p-Zap70 Y319 (271; CST), p-Lat Y191 (3584; CST), anti-Zap70 (2705; CST), anti-LAT (9166; CST), anti-SLP76 (4958; CST), anti-PLCγ1 (05–163; Millipore), anti-V5 (13202;CST), or anti-actin (ab3280; Abcam) antibodies and subsequently incubated with HRP-conjugated anti-rabbit or anti-mouse IgG (CST) in TBS containing 5% nonfat dry milk and 0.05% Tween 20. .. The final reaction was developed with a chemiluminescent system (Pierce).

    Western Blot:

    Article Title: Kinetic proofreading through the multi-step activation of the ZAP70 kinase underlies early T cell ligand discrimination.
    Article Snippet: .. The following antibodies were used for immunoblot analysis: anti-SLP76 (Cell Signaling Technology, catalog no. 4958), anti-ZAP70 (Cell Signaling Technology, catalog no. 2705, clone 99F2), anti-ZAP70-pY318 (Cell Signaling Technology, catalog no. 2701), anti-ZAP70-pY492 (Cell Signaling Technology, catalog no. 2704), anti-4E-BP1-pT37/T45 (Cell Signaling Technology, catalog no. 2855, clone 234B4), anti-PLCγ1-pY783 (Cell Signaling Technology, catalog no. 2821), anti-PLCγ1 (Cell Signaling Technology, catalog NATuRE IMMuNOLOGy | www.nature.com/natureimmunology ArticlesNATurE ImmuNOLOgy no. 2822), anti-LAT-pY220 (Cell Signaling Technology, catalog no. 20172, clone E3S5L), anti-LAT-pY255 (Cell Signaling Technology, catalog no. 45170), anti-ERK1/2-pY204/T202 (Cell Signaling Technology, catalog no. 9106, clone E10), anti-ERK1/2 (Cell Signaling Technology, catalog no. 9102), anti-FOXO3-pS252 (Cell Signaling Technology, catalog no. 13129, clone D18H8), anti-FOXO3 (Cell Signaling Technology, catalog no. 12829, clone D19A7), anti-SHC1-pY317/423 (Cell Signaling Technology, catalog no. 2431), anti-RPS6-pS235/ 236 (Cell Signaling Technology, cat 4858, clone D57.2.2E), anti-P70S6K-pT389 (Cell Signaling Technology, catalog no. 9206, clone 9206), anti-p90RSK1-S369 (Cell Signaling Technology, catalog no. 12032, clone D5D8), phospho-AKT substrates (Cell Signaling Technology, catalog no. 9611), anti-ZAP70-Y290 (Biolegend, catalog no. 691902, clone A16038A), anti-PDCD4- pS457 (Thermo Fisher, catalog no. PA5-38806), anti-LAT-pY132 (Thermo Fisher, catalog no. 44-224), anti-CD6 from (Novus Biologicals, catalog no. MAB727, clone 96123) and global anti-pY (Millipore, catalog no. 16-105, clone 4G10). ..

    Article Title: mDia1/3-dependent actin polymerization spatiotemporally controls LAT phosphorylation by Zap70 at the immune synapse
    Article Snippet: FITC-conjugated antibody to mouse CD4 (11-0042-81A) and PE-conjugated antibody to mouse CD3ε (12-0031-81; 145-2C11) were from eBioscience. .. The following antibodies were used for western blotting: anti-pZap70 [Y319] (2719), anti-Zap70 (3165), anti-pLAT [Y191] (3584), anti-LAT (9166) and anti-SLP76 (4985) from Cell Signaling Technology, anti-pSLP76 [Y128] (558367) from BD Pharmingen, anti-mDia1 (610849) from BD transduction, anti-mDia3 (HPA005647) from Sigma-Aldrich, anti-GAPDH (AM4300) from Ambion, HRP-conjugated anti-mouse IgG (NA931V) and HRP-conjugated anti-rabbit IgG (NA934V) from GE Healthcare. .. The following antibodies were used for immunocytochemistry: anti-mDia1 (610849) from BD transduction, anti-pZap70 [Y319] (2719), anti-pLAT [Y171] (3581), and Alexa555-conjugated anti-pZap70 [Y319] (custom order) from Cell Signaling Technology, Alexa 488-conjugated goat anti-mouse IgG (A11001) from Molecular Probes, and Cy2-conjugated donkey anti-mouse IgG (715-225-151), Cy3-conjugated goat anti-mouse IgG (115-166-003), Cy2-conjugated goat anti-rabbit IgG (111-225-003) and Cy3-conjugated donkey anti-rabbit IgG (711-165-152) from Jackson ImmunoResearch.

    Article Title: Kinetic proofreading through the multi-step activation of the ZAP70 kinase underlies early T cell ligand discrimination
    Article Snippet: .. The following antibodies were used for immunoblot analysis: anti-SLP76 (Cell Signaling Technology, catalog no. 4958), anti-ZAP70 (Cell Signaling Technology, catalog no. 2705, clone 99F2), anti-ZAP70-pY318 (Cell Signaling Technology, catalog no. 2701), anti-ZAP70-pY492 (Cell Signaling Technology, catalog no. 2704), anti-4E-BP1-pT37/T45 (Cell Signaling Technology, catalog no. 2855, clone 234B4), anti-PLCγ1-pY783 (Cell Signaling Technology, catalog no. 2821), anti-PLCγ1 (Cell Signaling Technology, catalog no. 2822), anti-LAT-pY220 (Cell Signaling Technology, catalog no. 20172, clone E3S5L), anti-LAT-pY255 (Cell Signaling Technology, catalog no. 45170), anti-ERK1/2-pY204/T202 (Cell Signaling Technology, catalog no. 9106, clone E10), anti-ERK1/2 (Cell Signaling Technology, catalog no. 9102), anti-FOXO3-pS252 (Cell Signaling Technology, catalog no. 13129, clone D18H8), anti-FOXO3 (Cell Signaling Technology, catalog no. 12829, clone D19A7), anti-SHC1-pY317/423 (Cell Signaling Technology, catalog no. 2431), anti-RPS6-pS235/ 236 (Cell Signaling Technology, cat 4858, clone D57.2.2E), anti-P70S6K-pT389 (Cell Signaling Technology, catalog no. 9206, clone 9206), anti-p90RSK1-S369 (Cell Signaling Technology, catalog no. 12032, clone D5D8), phospho-AKT substrates (Cell Signaling Technology, catalog no. 9611), anti-ZAP70-Y290 (Biolegend, catalog no. 691902, clone A16038A), anti-PDCD4- pS457 (Thermo Fisher, catalog no. PA5-38806), anti-LAT-pY132 (Thermo Fisher, catalog no. 44-224), anti-CD6 from (Novus Biologicals, catalog no. MAB727, clone 96123) and global anti-pY (Millipore, catalog no. 16-105, clone 4G10). ..



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